Jeffrey S. Grinstead

University of Puget Sound

Chemistry Department

Assistant Professor

1500 N. Warner, #1015

Tacoma, WA 98416

(253) 879-1571

jgrinstead@pugetsound.edu

Education:

Ph.D.University of Washington, Medicinal Chemistry (via the Biomolecular Structure and Design Program), 1997-2003

Structural Immunology of Humoral and Cellular Recognition of a MUC1 Breast Cancer Antigen

Thesis Advisor: A. Patricia Campbell

B.S.University of Puget Sound, Chemistry, 1993-199

Thesis Advisor: William Dasher

Teaching and Research Experience:

Assistant Professor, University of Puget Sound, 2008-present.  Chem460, 461, 110, 111, 230.

Visiting Assistant Professor, University of Puget Sound, 2007-2008. Teaching Chem460, Chem461 and Chem111.

Postdoctoral Research, Utrecht University, Bijvoet Center for Biomolecular Research, 2004-2007. Structural  and functional studies on AppA, a transcriptional regulator integrating light and redox signaling pathways in Rhodobacter Sphaeroides.

Research Advisors: Rolf Boelens and Robert Kaptein

Postdoctoral Research, University of Washington, Department of Medicinal Chemistry, 2003. Monitoring allostery in drug binding by cytochromes P450 using NMR spectroscopy.

Research Advisors: William M. Atkins and A. Patricia Campbell

Doctoral Thesis, University of Washington, Department of Medicinal Chemistry, 1998-2003. Structural immunology of humoral and cellular recognition of a MUC1 breast cancer antigen.

Thesis Advisor: A. Patricia Campbell

Publications:

Tripsianes, K., Folkers, G.E., Zheng, C., Das, D., Grinstead, J.S., Kaptein, R., and Boelens, R. (2007) Analysis of the XPA and ssDNA binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization, Nucleic Acids Research, 2007;35(17):5789-98 (Tripsianes_XPA.pdf).

Gauden, M., Grinstead, J.S., Laan, W., van Stokkum, I.H.M., Avila-Perez, M., Toh, K.C., Boelens, R., Kaptein, R., Grondelle, R.V., Hellingwerf, K.J., Kennis, J.T.M. (2007) On the role of aromatic sidechains in the photoactivation of BLUF domains, Biochemistry 46, 7405-7415 (Gauden_aromatic.pdf).

Grinstead, J.S., Avila-Perez, M., Hellingwerf, K.J., Boelens, R., Kaptein, R. (2006) Light-induced flipping of a conserved glutamine sidechain and its orientation in the AppA BLUF domain, J. Am Chem. Soc. 128, 15066-15067 (Grinstead_flip.pdf). 

Roberts, A.G., Diaz, M.D., Lampe, J.N., Shireman, L.M., Grinstead, J.S., Dabrowski, M.J., Pearson, J.T., Bowman, M.K., Atkins, W.M., and Campbell, A.P. (2006) NMR studies of ligand binding to P450(eryF) provide insight into the mechanism of cooperativity, Biochemistry 45 (6), 1673-84 (Roberts_eryF.pdf).

Grinstead, J.S., Hsu, S.-T. D., Laan, W., Bonvin, A.M.J.J., Hellingwerf, K.J., Boelens, R., and Kaptein, R. (2006) The solution structure of the AppA BLUF domain: insight into the mechanism of light-induced signaling, ChemBioChem 7 (1), 187-193 (file). 

Schuman, J.T., Grinstead, J.S., and Campbell, A.P. (2005) Structural and dynamic consequences of increasing repeats in a MUC1 peptide tumor antigen, Biopolymers 77 (2), 107-120. 

Grinstead, J.S., Schuman, J.T., and Campbell, A.P. (2003) Epitope mapping of antigenic MUC1 peptides to breast cancer antibody fragment B27.29: a heteronuclear NMR study, Biochemistry 42 (48), 14293-14305 (Grinstead_pepmap.pdf). 

Grinstead, J.S., Koganty, R.R., Krantz, M.J., Longenecker, B.M., and Campbell, A.P., (2002) Effect of glycosylation on MUC1 humoral immune recognition: NMR studies of MUC1 glycopeptide-antibody interactions, Biochemistry 41 (31), 9946-9961 (Grinstead_glyco.pdf). 

Storch, E.M., Grinstead, J.S., Campbell, A.P., Daggett, V., and Atkins, W.M. (1999) Engineering out motion: a surface disulfide bond alters the mobility of tryptophan 22 in cytochrome b5 as probed by time-resolved fluorescence and 1H NMR experiments, Biochemistry 38 (16), 5065-5075 (Storch_b5.pdf).